The course will focus on the following topics:
Generation of transgenic animals using the mouse system as an invaluable genetic tool:
-genetic manipulation of embryos and embryonic stem cells by using recombinant DNA molecules and viral vectors;
-embryonic stem (ES), primordial germ (PG) and induced pluripotent stem (iPS) cells;
-knock-out, knock-in and knock-down
animals;
-conditional transgenic organisms designed
to inactivate, express and silence genes
in an inducible manner (tissue-specific or time-specific);
-gene-trapping and genome-wide screenings
by RNAi libraries;
-mouse “disease model”;
Nuclear transfer technology, genome
reprogramming and chromatin remodeling;
Genome Editing: zinc finger nucleases, TALEN
and CRISPR;
Various transgenic models Drosophila,
C. Elegans and
Zebrafish
Use
of large size transgenic animals as
bioreactor to produce large-scale molecules.- Docente: DONATA ORIOLI